WDF Dye for Sysmex 5-Part Hematology Analyzers is a specialized fluorescent reagent formulated for automated optical flow cytometry, penetrating permeabilized white blood cell membranes to bind cellular nucleic acids for differential cell counting and scattergram separation.
What Are Hematology Stain / Dye Reagents?
Automated hematology dye reagents are specialized fluorophore solutions engineered for optical detection channels inside multi-parameter cell counters. Unlike manual blood smear stains used in conventional microscopy, these reagents contain synthetic fluorescent compounds dissolved in organic solvent carriers. During automated cell preparation, the reagent enters lysed cells to bind intracellular RNA and DNA, producing specific fluorescence signals under laser excitation.
Role in Cell Classification / Detection
Fluorescent dye reagents enable multi-dimensional cell identification by measuring light scatter and fluorescence intensity inside the flow cell.
Nucleic Acid Binding: The fluorophore binds to intracellular RNA and DNA, emitting light intensity proportional to cellular nucleic acid content and metabolic activity.
Leukocyte Differential: Separates mature lymphocytes, monocytes, neutrophils, and eosinophils into distinct clusters on two-dimensional scattergrams.
Abnormal Cell Flagging: Identifies atypical lymphocytes and immature cells based on elevated fluorescent signals from higher RNA and DNA concentrations.
Detection Channel
Automated 5-part hematology analyzers utilize discrete fluorescent reaction channels to process specific cell populations:
WDF Channel: Measures white blood cell differential counts, atypical lymphocytes, and immature granulocytes using side fluorescence and side scatter.
WNR Channel: Analyzes total white blood cell counts and nucleated red blood cells by isolating nuclear fluorescence signals.
WPC Channel: Screens for precursor cells, abnormal lymphocytes, and progenitor cell populations.
RET Channel: Quantifies reticulocytes and mature erythrocytes by targeting intracellular ribosomal RNA.
PLT Channel: Provides optical platelet counts using fluorescent markers to prevent interference from cell fragments or microcytic erythrocytes.
Compatible Analyzer Series
Automated 5-Part Hematology Analyzers: Modular high-throughput platforms, standalone floor-standing units, and compact benchtop systems.
Matching Product Identifiers: STAC-DYE-SYSWNR, STAC-DYE-SYSWDF, STAC-DYE-SYSWPC, STAC-DYE-SYSRET, STAC-DYE-SYSPLT
Product Specifications
|
Parameter |
Specification |
|
Product Model |
STAC-DYE-SYSWDF |
|
Target Channel |
WDF (White Blood Cell Differential) |
|
Active Molecule |
Polymethine Fluorescent Compound |
|
Excitation / Emission Wavelength |
633–635 nm / 650–670 nm |
|
pH Range |
7.00–7.40 at 25°C |
|
Precision (CV) |
WBC ≤ 3.0%, NEUT% ≤ 5.0%, LYMPH% ≤ 5.0% |
|
Correlation ($R^2$) |
≥ 0.99 |
|
Shelf Life / Open Vial Stability |
18 Months (Unopened) / 60 Days (Opened) at 2°C–35°C |
Application & Reagent Function
Routine 5-Part Leukocyte Differential: Stains intracellular nucleic acids to separate lymphocytes, monocytes, neutrophils, and eosinophils into distinct scattergram clusters.
Immature Granulocyte Quantification: Generates distinct fluorescent intensity signals for precursor cells to enable automated counting without manual smear reviews.
Atypical Lymphocyte Detection: Identifies elevated RNA levels in reactive leukocytes to trigger automated analytical alerts.
Package / Storage
Storage Temperature: 2°C–35°C. Store in original dark containers away from direct light.
Container Specifications: High-density polyethylene amber bottles featuring induction-sealed closures.
Transport Conditions: Certified for ambient freight transport. Protect from freezing conditions below 0°C.
Related Hematology Reagents
STAC-DYE-SYSWNR: Fluorescent dye reagent for total white blood cell count and nucleated red blood cell analysis.
STAC-DYE-SYSWPC: High-specificity fluorescent stain reagent for precursor cell and abnormal leukocyte screening.
STAC-DYE-SYSRET: Fluorescent nucleic acid stain for reticulocyte fraction quantification.
STAC-DYE-SYSPLT: Optical fluorescent dye reagent for precise platelet determination in complex samples.
FAQ
Q: How does WDF dye prevent signal overlap between neutrophils and eosinophils on scattergrams?
A: The formulation works together with compatible lyse reagents that selectively alter cell membranes. The dye intercalates into nuclear DNA and cytoplasmic RNA, generating distinct side fluorescence and side scatter intensity coordinates for each cell population.
Q: What is the stability duration for open reagent containers?
A: Open containers remain stable for 60 days when kept between 2°C and 35°C. The solvent formulation prevents active compound degradation and solvent evaporation under standard laboratory humidity conditions.
Q: Can these dye reagents be installed into fluidic lines without fluidic adjustments?
A: Yes. The reagent maintains matched fluidic viscosity, laser absorption profiles, and operational parameters, allowing direct connection to matching flow channels.
Q: How is batch-to-batch fluorescent stability monitored during manufacturing?
A: Liquid chromatography controls verify active molecule purity for every production batch, followed by multi-channel flow testing to maintain scattergram cluster positioning drift within ≤ 2.0%.
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