This WNR Dye for Sysmex 5-Part Hematology Analyzers is formulated for automated 5-part differential blood counters using laser fluorescence flow cytometry, enabling rapid cell membrane permeation to precisely count white blood cells, basophils, and nucleated red blood cells.
What Are Hematology Stain / Dye Reagents?
Automated hematology stain and dye reagents are fluorochrome-based chemical solutions used in automated 5-part differential blood counters. Unlike manual staining dyes used in microscopy, these reagents bind specifically to nucleic acids (RNA and DNA) within intact or lysing cells inside automated flow cytometry fluidic channels.
Role in Cell Classification / Detection
In automated flow cytometry channels, the dye permeates cell membranes and binds to intracellular nucleic acids. When exposed to a red semiconductor laser beam, the dye fluoresces, emitting light intensity directly proportional to nucleic acid content. Combined with forward-scattered light measuring cell size and side-scattered light measuring internal complexity, fluorescence intensity differentiates distinct cell populations on two-dimensional scattergrams.
Detection Channel
The WNR (White Cell Nucleated) channel utilizes this dye in combination with a lysing reagent to achieve three precise analytical functions:
Total white blood cell enumeration
Basophil separation based on differential cell lysis resistance and fluorescence intensity
Nucleated red blood cell identification without mature erythrocyte interference
Compatible Analyzer Series
XN Series
XN-L Series
XS Series
XT Series
XE Series
Product Specifications
|
Parameter |
Specification |
|
Catalog Code |
STAC-DYE-SYSWNR |
|
Methodology |
Semiconductor Laser Fluorescence Flow Cytometry |
|
Target Channel |
WNR Channel |
|
Measured Analytes |
WBC, BASO (#, %), NRBC (#, %) |
|
Physical Form |
Clear liquid dye solution |
|
Excitation Wavelength |
633 nm – 635 nm |
|
Background Signal |
WBC <= 0.05 x 10^9/L |
|
Lot Precision (CV%) |
<= 2.0% |
|
Correlation (r) |
>= 0.98 |
|
Shelf Life |
18 Months (2°C - 30°C) |
|
Open-Vial Stability |
60 Days on-board |
Application & Reagent Function
Automated Differential Counting: Delivers high-volume leukocyte counting and differential analysis in centralized clinical laboratories.
NRBC Quantification: Measures nucleated red blood cells to prevent false-positive leukocyte counts without requiring manual blood smear reviews.
Basophil Resolution: Provides distinct basophil cluster separation on scattergrams across low-concentration patient specimens.
Package / Storage
Storage Temperature: 2°C to 30°C (35.6°F to 86°F). Do not freeze. Store away from direct light.
Packaging Design: Sealed fluidic cartridges with anti-evaporation caps for direct fluid connection.
Transit Stability: Insulated export packaging prevents thermal degradation during international transport.
Related Hematology Reagents
STAC-DYE-SYSWDF: Differential dye reagent for WBC 4-part differentiation in the WDF channel.
STAC-DYE-SYSWPC: Pre-diluent dye reagent for precursor and abnormal cell detection in the WPC channel.
STAC-DYE-SYSRET: Reticulocyte dye reagent for RNA labeling in the RET channel.
STAC-DYE-SYSPLT: Optical platelet dye reagent for platelet counts in the PLT-F channel.
FAQ
Q: Is this dye reagent compatible with automated fluidic systems without physical modification?
A: Yes, the packaging and connector interfaces match standard automated analyzer fluid line setups, allowing direct installation without instrument adjustments.
Q: How is lot-to-lot signal consistency maintained across different production batches?
A: Each production lot undergoes raw material fluorochrome purity testing, standardized formulation control, and automated flow cytometry calibration verification against analytical controls to maintain a coefficient of variation under 2.0%.
Q: What are the transportation conditions for international shipping?
A: The reagent is packaged in insulated containers designed to maintain temperatures between 2°C and 30°C during transit, protecting fluorochrome integrity against temperature extremes.
Q: What is the open-vial stability once installed on the analyzer?
A: Once connected to the system, the reagent remains stable on-board for 60 days, preventing signal degradation and fluidic evaporation over standard operating cycles.
Q: What documentation is provided for batch verification and regulatory compliance?
A: Every shipment includes a Lot-Specific Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), ISO 13485 documentation, and CE compliance certification.
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